CD Genomics Explores the Difference Between 16S Sequencing and 16s Metagenomics Sequencing

Share This Post

New York, NY - August 11, 2016 - (

I have been wondering: what are the similarities and differences between 16S sequencing and 16s metagenomics sequencing? Why can 16S only do function prediction, and the metagenomics can do functional annotation?

In order to find the answer, today CD Genomics shares the esoterica to compare those two sequencing.

16S rDNA sequencing: it refers to the PCR amplification for a certain period in a sample of 16S rDNA by using high-throughput sequencing instruments. It mainly identifies the microbial species and abundance of the bacteria in a specific environment.

16s metagenomics sequencing: it is the sum of all the genetic material in the habitat. With the development of high-throughput sequencing technology, the metagenomics technology has been greatly developed, especially in the application of human intestinal microorganisms. Metagenomic sequencing method avoids the defects of microbial cultivation, greatly broadening the use space of microbial resources, which becomes one of the most important issues in life science research.

Research contents

16S rdna analysis:

Species notes

Species richness

System evolution

Metagenomic analysis:

Species notes

Species richness

System evolution

Gene abundance

Functional gene annotation and analysis

The way of building

16S rDNA library sequencing process:

DNA quality inspection—primer design and synthesis—PCR amplification and product purification—PCR product quantification and homogenization—Illumina high throughput sequencing

Metagenomic library sequencing process:

DNA quality inspection—DNA fragment—library construction—cluster generation—Illumina high throughput sequencing

Sequence assembly

16S sequence stitching principle: forward sequencing reads and reverse sequencing reads according to the site of overlap

Stitching software: PANDAseq

The principle of metagenomic stitching: based on the principle of De-Brujin graph by using different sizes of K-mer to assemble the filtered data.

Stitching software: SOAPdenovo

Content analysis

1.Species notes

16S species notes: compared to RDF database based on OTU

Metagenomic species notes: comared to reference database of known bacteria, fungi and viruses in NCBI database based on the Clean Reads

2.Species comparison

The distribution of species composition is different in 16S and metagenomic sequencing.

3.OTU of 16S and the gene of metagenomics

16S is based on the OTU abundance table, the species diversity of Alpha, Beta diversity, the difference between groups and so on.

Metagenomics is based on gene abundance to do CAG analysis, MGS analysis, functional annotation and other analyses.

4.Functional analysis

16S function prediction

metagenomics function analysis

Related Links
sequenom massarray

Press Release Service by

Original Source: CD Genomics Explores the Difference Between 16S Sequencing and 16s Metagenomics Sequencing

Related Posts

VIP Minds CEO and Visionary Nora Abou Chakra Launches ‘Power Hearts,’ a Transformative Initiative Driving Social Change

CEOs revolutionizing philanthropy through inspiring charitable actions

Today marks the launch of "Power Hearts," an innovative initiative led by businesswoman and visionary Nora Abou Chakra. With each endeavor, Power Hearts embarks on a different mission, empowering CEOs to take direct action and make a tangible impact. Through immersive experiences and hands-on involvement, Power Hearts addresses pressing societal issues, fostering empathy and inspiring CEOs to become advocates for change within their organizations and communities.

Nora Abou Chakra, a respected entrepreneur known for her philanthropic endeavors, has once again demonstrated her commitment to driving social change with the launch of Power Hearts. Under this empowering initiative, CEOs from diverse industries come together to tackle pressing issues and create transformative solutions.

An example of these missions is hunger. Power Hearts combats hunger and alleviate the suffering of those affected by it. CEOs gather in a large-scale communal kitchen, where they actively participate in purchasing groceries and cooking meals for those in need.

The immersive experience provided by Power Hearts goes beyond the kitchen. CEOs physically hit the streets, personally distributing the freshly prepared meals to individuals experiencing hunger. This direct interaction with those in need further deepens their understanding of the issue, sparking a profound connection and a sense of shared humanity.

CEOs return to their organizations with a renewed perspective and a profound commitment to addressing the issue of hunger. They become ambassadors for change, leveraging their influence and resources to advocate for hunger relief initiatives within their respective communities and among their staff members.

Nora Abou Chakra, the driving force behind Power Hearts, expressed her enthusiasm for the initiative, stating, "Power Hearts aims to create a ripple effect of compassion and advocacy. By immersing CEOs in the realities of pressing social issues, we ignite a powerful drive for change that reverberates throughout their personal and professional spheres. Together, we can make a significant difference and create a more compassionate and equitable world."

Power Hearts represents Nora Abou Chakra's unwavering commitment to leveraging the influence and resources of business leaders for the greater good. By providing CEOs with transformative experiences, Power Hearts empowers them to become catalysts for change and advocates for social causes that resonate with their hearts.

For more information about Power Hearts and its upcoming initiatives, please visit or follow us on @power.hearts

Contact Information:
Stephanie Khalil
Executive Assistant to CEO

Original Source: VIP Minds CEO and Visionary Nora Abou Chakra Launches 'Power Hearts,' a Transformative Initiative Driving Social Change

NAACP Excellence in Teaching Award Winner Andee Nunn Releases Memoir, ‘Magic in Room 216’, on Cyber Monday

Profound Education and Inspirational Life Journey Enshrines Era of Inclusion DANBURY, Conn., November 27, 2023 (New...
green agriculture project
- Part of VUGA Media group -best seo company